ABSTRACT
Objective To investigate the expression of neutrophil extracellular traps ( NETs) in ulcerative colitis mouse model and its correlation with the degree of intestinal barrier function. Methods Sixty male Balb/c mice were randomly divided into normal group( n＝30) and model group( n＝30). An ulcerative colitis model was established by dextran sulfate sodium,and intestinal tissue and blood samples were taken on day 1,day 3,day 5 after modeling(10 mice at each time point). By observing the disease activity index and HE pathological analysis,the intestinal tissue damage was reflected. The cell free DNA was quantified using PicoGreen?dye,and the expression and localization of myeloperoxidase and citrullinated his-tone in the intestine were observed by immunohistochemistry. Western blot was used to detect the expression of Claudin-1. Results Compared with the normal group,the disease activity index in model group increased significantly (P<0. 05). The content of cell free DNA increased significantly ( P <0. 05). Immunohisto-chemical examination showed that the formation of NETs in the model group increased,and with the disease recovers, its content gradually decreased. Western blot analysis showed that the expression of claudin-1 in the model group decreased (P<0. 05),and with the disease recovers, its content gradually increased. Conclu-sion The formation of NETs increases in the mouse model of ulcerative colitis,and the increased formation of NETs is associated with impairment of intestinal barrier function.
ABSTRACT
Inflammatory bowel disease(IBD) is a group of chronic inflammatory conditions of the gastrointestinal tract that includes two major phenotypes,Crohn's disease and ulcerative colitis.The exact etiology of IBD still remains unknown,although it is thought that the diseases result from an excessive immune response directed against microbial or environmentally derived antigens which can be triggered by the disruption of the intestinal epithelial barrier integrity.Innate immunity is the fast line of defense against the invasion of various pathogenic microorganisms.The innate immune cells,including neutrophils,macrophages,dendritic cells and innate lymphocytes,play an important role in the occurrence,development and prognosis of IBD.In this paper,we review the research progress on the role of innate immune cells in the pathogenesis of IBD in order to provide new ideas for the pathogenesis research and clinical treatment of IBD.